First study to show that microginins are genotoxic

Abstract from a paper by Ujvarosi et al. (2019), published in Chemospere :

Microginins (MGs) are bioactive metabolites mainly produced by Microcystis spp., (Cyanobacteria) commonly found in eutrophic environments. In this study, the cytotoxic and genotoxic activities of four MG congeners (MG FR3, MG GH787, cyanostatin B, MGL 402) and a well characterized cyanobacterial extract B-14-01 containing these metabolites were evaluated in the human hepatocellular carcinoma (HepG2) cell line. The cytotoxicity was measured with the MTT assay, while genotoxicity was studied with the comet, γH2AX and cytokinesis block (CBMN) micronucleus assays. The viability of cells after 24 h was significantly affected only by the extract, whereas after 72 h a concentration dependent decrease in cell proliferation was observed for the extract and tested microginins, with MGL 402 being the most potent and MG FR3 the least potent congener. The extract and all tested congeners induced DNA strand breaks after 4 and 24 h exposure. The most potent was the extract, which induced concentration and time dependent increase in DNA damage at concentrations ≥0.01 μg mL−1. Among microginins the most potent was MGL 402 (increase in DNA strand breaks at ≥ 0.01 μg mL−1) and MG FR3 was the least potent (increase in DNA strand breaks at ≥ 1 μg mL−1). However, no induction of DNA double strand breaks was observed after 24 and 72-h exposure to the cyanobacterial extract or MGs. Induction of genomic instability was observed in cells exposed to MG GH787, cyanostatin B and the extract B-14-01. This study is the first to provide the evidence that microginins exert genotoxic activity.

The paper is a product of joined research by groups in Slovenia and Hungary and features CYANOCOST members Bojana Zegura, Gabor Vasas, Klara Hercog, Metka Filipic. The authors acknowledge CYANOCOST.

Reference:

Andrea Zsuzsanna Ujvárosi, Klara Hercog, Milán Riba, Sándor Gonda, Metka Filipič, Gábor Vasas, Bojana Žegura (2019). “The cyanobacterial oligopeptides microginins induce DNA damage in the human hepatocellular carcinoma (HepG2) cell line”, Chemosphere, Volume 240,  https://doi.org/10.1016/j.chemosphere.2019.124880.

 

PhD position at the Research Department for Limnology Mondsee (University of Innsbruck) – Interreg (Alpine Space) project “Eco-AlpsWater”

The aim of the project Eco-AlpsWater is to improve the traditional monitoring approaches used in the Alpine region (Water Framework Directive 2000/60/EC-EU WFD and, in Switzerland, the Water Protection Ordinance-WPO) with advanced and innovative DNA sequencing techniques such as metabarcoding. The new approach will make use of Next Generation Sequencing (NGS) to analyze environmental DNA in waterbodies (which allow rapid species identification at low costs, from fish to bacteria), and smart technologies (automation in data processing, data storage, information retrieval). Along with the identification of gaps in the monitoring approaches across the Alpine regions, the new technologies will allow to define improved experimental monitoring protocols to be applied in pilot areas partly linked to other ongoing alpine space projects (SPARE, Hymocares) and including large perialpine lakes as well as smaller waterbodies, and rivers.

The position will be financed for 3 years (30 working hours per week, 75 percent appointment) according to the collective labour agreement (Kollektivvertrag) of the Austrian Universities (2096.- Euro monthly salary, 14x per year). We are looking for a person holding a Master degree in Biology with experience in limnology (or comparable qualification), dedicated to apply metabarcoding approaches in fish communities (respectively other organism groups such as planktonic and benthic algae). Parts of the working activities (e.g. sampling and laboratory analysis) will be performed with partner organisations of the project, i.e. in Italy, Switzerland, France, Germany or Slovenia. Further activities will comprise metabarcoding data analysis, report writing, preparation of scientific manuscripts, as well as communication with project partners and observers. The registration of the PhD thesis in Biology at the University of Innsbruck and the administration within the new doctoral programme “Alpine Biology and Global Change” will be expected.

Further Information: Univ. Doz. Dr. Josef Wanzenböck & Assoc. Prof. Dr. Rainer Kurmayer, Research Department for Limnology Mondsee, University of Innsbruck

http://www.uibk.ac.at/limno/index.html.de

email: Josef.wanzenboeck@uibk.ac.at; rainer.kurmayer@uibk.ac.at. For application please send a motivation letter together with a CV (in English).

Download in pdf format.