A new paper by Stern et al., published in Food and Chemical Toxicology, provides evidence for ROS-mediated genotoxic effects of Nodularin. From the abstract:
“The cyanobacterial pentapeptide nodularin (NOD), mainly produced by genus Nodularia, is a potent inhibitor of protein phosphatases PP1 and PP2A, and causes animal mortality. The few studies available indicate that NOD is a potential non-genotoxic carcinogen. In the present study we evaluated NOD (0.01, 0.1 and 1 μg/ml) genotoxic activity in human hepatoma (HepG2) cells with the comet, γH2AX and cytokinesis block micronucleus cytome assays. In addition, induction of oxidative stress was studied. Moreover changes in the expression of selected genes from the P53 pathway, involved in the response to DNA damage (P53, GADD45α, CDKN1A, MDM2), apoptosis (BAX, BCL2) and oxidative stress (GPX1, GSR, GCLC, CAT, SOD1) were determined using qPCR. Non-cytotoxic concentrations induced time and dose dependant increase in reactive oxygen species (ROS) production and substantially increased the formation of oxidative DNA damage. In addition, elevated formation of micronuclei was detected. For the first time it has been shown that NOD deregulated the mRNA level of DNA damage (CDKN1A, GADD45α) and oxidative stress (GPX1, GSR, GCLC, CAT and SOD1) responsive genes and anti-apoptotic gene BCL2. Our results provide new evidence that NOD genotoxic effects are mediated through ROS production, already at low environmentally relevant concentrations.”
The paper acknowledges CYANOCOST.
A. Štern, A. Rotter, M. Novak, M. Filipič, B. Žegura (2019). Genotoxic effects of the cyanobacterial pentapeptide nodularin in HepG2 cells. Food and Chemical Toxicology 124, 349-358. https://doi.org/10.1016/j.fct.2018.12.019
The 19th Congress of the European Section of the International Society of Toxinology (IST) will be held on 22-26 Sept. 2018 in Yerevan, Armenia. The congress will include updates on all aspects of toxinology – from evolution and molecular biology, through chemistry and pharmacology, to clinical effects and advances in treatments of envenomed patients.
The closing date for the early-bird registration rate is 30 June, and the closing dates for submission of abstracts are 1 July for oral presentations and 15 August for posters.
Further details of the congress can be found at www.ist2018.sci.am
Invitation from the Local Organizing Committee of ICTC11 (from ICTC11 website):
The International Conference on Toxic Cyanobacteria (ICTC) is a periodic summit of an international community focusing on the study of cyanotoxins and toxic cyanobacteria. Poland was chosen as the venue of the next ICTC11 by the participants of the conference in Wuhan in 2016. The five-day event includes interactive sessions and lively discussion panels which promote active scientific exchange and communication between scientists and students. This event will feature recent findings from leading academic experts in the field in the form of lectures and posters. Participants will have the opportunity to gain hands-on experience from the experts on the subject and to network with other enthusiasts.
The theme of this year’s ICTC 11 is: “Learning from the past to predict the future”
The event also includes excursions and off-site visits with the aim of giving the participants a wider insight about the heritage of Kraków and Małopolska region. The royal capital city of Kraków is the spiritual centre of Poland with rich heritage. Krakow’s Old Town, along with Wawel Castle and the city’s Kazimierz district found their place on the First World Heritage List, created by UNESCO in 1978. The city is home to Polish intellectual and artistic elites and is a magnet for the young. Students and young Polish professionals as well as a growing international community are drawn to the city.
On behalf of the Local Organizing Committee, we cordially invite you to attend the 11th International Conference on Toxic Cyanobacteria to be held in Kraków, Poland from 5 to 10 May, 2019.
Dr. Dariusz Dziga
On behalf of the Local Organizing Committee
The American Chemical Society – Division of Environmental Chemistry (ACSenvr) is organizing a “Satellite Event” (1-day workshop) within the ICCE17 conference in Oslo, on 18 June 2017. The workshop is titled “Algae toxins: Methods and challenges”. Please see the Event’s Flyer for more details (it contains links to the website of ICCE17).
We warmly invite you to participate in this workshop and present your work in one of the four topics/sessions.
If you are willing to participate could you please send a provisional title and indication of the topic that fits to your presentation by e-mail to Tri Kaloudis. This will help us prepare the program.
Please notice that formal submission of abstracts will be done according to submission procedure of ICCE17 (by e-mail), where the Satellite Event “Algae Toxins: Methods and Challenges” must be indicated.
Deadline for submission of abstracts is 17 April 2017.
For this event, 1-day fees will apply. Some support to cover fees may be provided to presenters from ACSenvr, depending on the number of participants in the final program.
Could you also please distribute this information to your networks and anyone who may be interested.
The Fifth Joint Symposium and AOAC Task Force Meeting, was held in Baiona, Spain, on June 14-17 2015. The symposium was jointly organized by the Univesity of Vigo, AOAC and CYANOCOST. The Symposium Chair was Dr Ana Gago-Martinez of the Department of Analytical and Food Chemistry, University of Vigo and head of the European Reference Laboratory for Marine Biotoxins which is located in the campus of the University.
The Symposium addressed the state-of-the-art and advances on the field of the analysis of Marine and Freshwater toxins, with regards to new method development, validation and implementation. While previous editions of the Symposium concerned only marine toxins (e.g. saxitoxins, domoic acids, okadaic acids, azaspiracids etc), the 2015 Symposium focused also on freshwater cyanotoxins (e.g. microcystins, cylindrospermopsin, anatoxin, emerging toxins). This was achieve through co-organization with CYANOCOST, plenary presentations by CYANOCOST speakers, participation of CYANOCOST experts in AOAC task-force meeting and discussions between “marine” and “freshwater” experts.
The presentations are available online:
Vitor Vasconcelos (keynote): “Current and emerging methods for the analysis of cyanobacteria toxins: challenges and needs”
Shmuel Carmeli: “Non -Toxic Mediators of Cyanobacteria Toxic Metabolites – Do We Overlook Their Importance?”
Hanna Mazur-Marzec: “Toxins and other bioactive metabolites produced by the Baltic cyanobacteria”
Emanuela Testai: “Cyanotoxins as emerging contaminants in matrixes other than water and health implications”
Leonardo Cerasino: “Shift from microcystins dominance to anatoxin-a dominance in the cyanotoxin population of lake garda”
Triantafyllos Kaloudis: “Laboratory accreditation of freshwater cyanotoxin analysis. Current status, gaps and future prospects”
All the Symposium presentations can be found here.